Future studies aimed at identifying additional genes associated with this plasmodesmal macromolecular trafficking system will advance our understanding of the function and evolution of this novel plant communication system. Trends Biochem. It is a microsurgical procedure conducted on a single cell, using a glass needle (i.e., a fine, glass microcapillary pipette), a precision positioning device (a micromanipulator) to control the movement of the micropipette, and a microinjector. a powerful experimental approach to elucidate the processes underlying plant growth and development. Annu. 47, 1119–1128. USA 87, 3284–3288. c. The … The application of microinjection methods, in conjunction with molecular tools, established a powerful experimental approach to elucidate the processes underlying plant growth and development. Plant Physiol. enable JavaScript in your browser. Proc. 73, 208–215. J. Exp. Science 275, 1298–1300. Rev. Olesinski A A, Almon E, Navot N, Galun E, Lucas W J and Wolf S 1996 Tissue specific expression of tobacco mosaic virus movement protein in transgenic potato plants alters plasmodesmal function and carbohydrate partitioning. Res. 3, 308–315. Cleland R E, Fujiwara T and Lucas W J 1994 Plasmodesmal-mediated cell-to-cell transport in wheat roots is modulated by anaerobic stress. Kühn C, Franceschi V R, Schulz A, Lemoine R and Frommer W B 1997 Macromolecular trafficking indicated by localization and turnover of sucrose transporters in enucleate sieve elements. USA 91, 1433–1437. II. This versatile volume updates established techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Annu. 32, 387–411. Ishiwatari Y, Fujiwara T, McFarland K C, Nemoto K, Hayashi H, Chino M and Lucas W J 1997 Rice phloem thioredoxin h has the capacity to mediate its own cell-to-cell transport through plasmodesmata. Plant Cell 4, 915–928. Noueiry A O, Lucas W J and Gilbertson R L 1994 Two Proteins of a plant DNA virus coordinate nuclear and plasmodesmal transport. and so many different model systems … associated with other master technique (e.g., western blot analysis). 18, 1301–1310. Especially in the field of male infertility, this groundbreaking application is widely used and the suspicion that anomalies will occur in the pregnancies that occur despite the birth of thousands of babies all over the world … Natl. Soviet Plant Physiol. 114, 305–340. Robards A W and Lucas W J 1990 Plasmodesmata. Annu. 387p. Tanner W and Caspari T 1996 Membrane transport carriers. Plant Mol. Figure 8. Ishiwatari, Y, Honda C, Kawashima I, Nakamura S, Hirano H, Mori S, Fujiwara T, Hayashi H and Chino M 1995 Thioredoxin h is one of the major proteins in rice phloem sap. Inverted microscopes are generally used for microinjection applications. ...you'll find more products in the shopping cart. Phytopathol. Editors: Balachandran S, Hull R J, Vaadia Y, Wolf S and Lucas W J 1995 Tobacco mosaic virus movement protein-induced change in carbon partitioning originates from the mesophyll and is independent of change in plasmodesnial size exclusion limit. You’ve just watched JoVE’s introduction to microinjection of early zebrafish embryos. The application of microinjection methods, in conjunction with molecular tools, established. Lucas W J 1995 Plasmodesmata: intercellular channels for macromolecular transport in plants. The microinjection polymer molding market growth is driven by demand for micro gears, replacing affordable feedstock like PEEK. 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